This study examines the developmental and transcriptomic responses of Hawaiian bobtail squid to ocean warming and acidification, finding trade-offs in metabolism versus development and molecular changes like alternative splicing.

Proceedings of the Royal Society B Biological Sciences · Dec 17, 2025Peer-reviewedEcology

Nanoscale imaging of native symbiotic animal tissue using amultimodal large volume imaging pipeline for cryo-electrontomography

Katrina A. Gundlach, Oda Helene Schiøtz, Mark S. Ladinsky, Colin Raimann, Michael Rheinberger, Florian Beck, Barış Gündüz, Rob Langelaan, Martin Rücklin, Ronald W.A.L. Limpens, Edward G. Ruby, Margaret McFall‐Ngai, Jürgen M. Plitzko, Ariane Briegel

The paper develops a multimodal imaging pipeline to enable cryo-ET of symbiotic tissues, revealing nanoscale structural details of host-microbe interactions in Hawaiian bobtail squid.

bioRxiv · Nov 30, 2025 · PreprintMicrobiology

Climate-driven warming disrupts the symbiosis of bobtail squid Euprymna scolopes and the luminous bacterium Vibrio fischeri

Eve Otjacques, Brandon Jatico, Tiago André Marques, José Carlos Xavier, Edward G. Ruby, Margaret McFall‐Ngai, Rui Rosa

This study examines how climate-driven warming affects the symbiotic relationship between the bobtail squid Euprymna scolopes and the bacterium Vibrio fischeri, finding reduced hatching success, lower survival, and diminished symbiosis capacity under higher temperatures.

Global Change Biology · May 1, 2025Peer-reviewedEcology

Application of hsp60 amplicon sequencing to characterize microbial communities associated with juvenile and adult Euprymna scolopes squid

Stephanie N. Smith, Clotilde Bongrand, Susannah Lawhorn, Edward G. Ruby, Alecia N. Septer

This paper uses hsp60 amplicon sequencing to characterize microbial communities in Euprymna scolopes squid, showing V. fischeri dominance in light organs and detecting other microbes in ventate samples.

ISME Communications · Jan 1, 2025Peer-reviewedMicrobiology