Jürgen M. Plitzko

Layered helical order reveals assembly states in podosome actin networks

The paper uses cryo-electron tomography and computational methods to infer the assembly states of actin networks in podosomes, revealing layered helical order and preferred orientation transitions.

In situ architecture of plasmodesmata suggests mechanisms controlling intercellular exchange

The paper uses cryo-electron tomography to study plasmodesmata structure in Physcomitrium patens, revealing callose's role in shaping channels and identifying MCTP protein assemblies.

Towards community-driven visual proteomics with large-scale cryo-electron tomography of Chlamydomonas reinhardtii

Automated cryogenic plasma-focused ion beam milling and cryo-electron tomography of Chlamydomonas reinhardtii yield 1,829 annotated cellular tomograms and sub-nanometer in situ structures of six macromolecular complexes.

Exploring shaped focused ion beams for lamella preparation

This paper explores the use of shaped focused ion beams for preparing TEM lamellae, demonstrating the feasibility of cellular lamella milling with an ion knife.

Nanoscale imaging of native symbiotic animal tissue using amultimodal large volume imaging pipeline for cryo-electrontomography

The paper develops a multimodal imaging pipeline to enable cryo-ET of symbiotic tissues, revealing nanoscale structural details of host-microbe interactions in Hawaiian bobtail squid.

The small GTPase Ran defines Nuclear Pore Complex asymmetry

This study shows that the small GTPase Ran controls the asymmetry of nuclear pore complexes by regulating their peripheral configuration in budding yeast and Drosophila.

Sub-3 A resolution protein structure determination by single-particle cryo-EM at 100 keV

This paper demonstrates that a 100 keV cryo-TEM with advanced components can achieve sub-3 Å resolution structures of various proteins and viruses.

The targeting of non-fibrillar polyQ via distinct VCP-proteasome coupling

This study investigates how VCP and proteasomes degrade non-fibrillar polyQ intermediates in neurodegenerative diseases, revealing a distinct VCP-20S coupling mechanism.

Making plant tissue accessible for cryo-electron tomography

This paper describes optimized cryo-ET sample preparation workflows for plant tissues, achieving subnanometer resolution through targeted FIB milling and lamella trimming.

Global cellular proteo-lipidomic profiling of diverse lysosomal storage disease mutants using nMOST

This study uses nMOST to profile proteo-lipidomics in lysosomal storage disease mutants, revealing autophagy defects and mitochondrial dysfunction.

Multishot Tomography for High-Resolution In Situ Subtomogram Averaging.

This paper introduces a fast multi-position tomographic acquisition method for subtomogram averaging, achieving sub-nanometer resolution in situ.

A cryogenic, coincident fluorescence, electron and ion beam microscope

This paper introduces a new cryogenic microscope that combines fluorescence, ion beam, and electron beam imaging to improve the targeting and fabrication of lamellae for high-resolution imaging.

Optimizing Cryo-FIB Lamellas for sub-5 Angstrom in situ Structural Biology

This paper introduces a method using metallic platinum sputtering to improve subtomogram averages, enabling sub-5 Angstrom resolution of yeast ribosomes with fewer particles.

A streamlined workflow for automated cryo focused ion beam milling

The paper introduces hardware and software improvements to enhance cryo-FIB milling for cryo-electron tomography.

Structure of a hibernating 100S ribosome reveals an inactive conformation of the ribosomal protein S1

The paper presents cryo-EM structures of hibernating 70S and 100S ribosomes from E. coli, revealing the roles of HPF and RMF in ribosome inactivation and dimerization.

Eukaryotically expressed encapsulins as orthogonal compartments for multiscale molecular imaging

The paper demonstrates the use of bacterial encapsulins in eukaryotic cells to create nanocompartments for localized enzymatic reactions and multiscale molecular imaging.