Author

Louis Robert Hollingsworth

Harvard UniversityORCID 0000-0002-4130-3580Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Assay:proteomics· 3 Research Focus:cell type annotation· 1 Research Focus:database / resource· 1 Research Focus:drug response / drug discovery· 1 Approach:experimental assay· 1 Organism:human· 1 Approach:large language model· 1 Approach:machine learning (classical)· 1

Frequent Co-Authors

J. Wade HarperiD· 2 shared Aaron W. KollaschiD· 1 shared Adam J. RiesselmaniD· 1 shared Andre A. R. TeixeiraiD· 1 shared Andrew C. KruseiD· 1 shared Artem GazizoviD· 1 shared Conor T. McMahoniD· 1 shared Debora S. MarksiD· 1 shared

Activity by Year

2024-2026 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 45 mutual co-author links

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2 shared papersBioinformatics

J. Wade HarperORCID

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Author profile →

3 papers

Recent·Most cited

The accessible human surfaceome

Rebecca Carlson, Louis Robert Hollingsworth, Michael J. Smallegan

The paper curates an accessible human surfaceome using an LLM pipeline, outperforming existing databases and identifying new candidate surface proteins.

bioRxiv · Oct 19, 2026 · PreprintBioinformatics

Machine Learning enables efficient and effective affinity maturation of nanobodies

Steffan B. Paul, Edward P. Harvey, James Osei‐Owusu, Aaron W. Kollasch, Adam J. Riesselman, Conor T. McMahon, Artem Gazizov, Murali Anuganti, Filmawit Belay, Minh Anh Kieu, Haisun Zhu, L. Robert Hollingsworth, J. Wade Harper, Deborah J. Moshinsky, Andre A. R. Teixeira, , Andrew C. Kruse

This paper uses machine learning to predict high-affinity nanobody binders from sparse sequencing data, outperforming deep learning and semi-supervised methods with linear models.

bioRxiv · Jan 12, 2026 · PreprintBioinformatics

Spatiotemporal proteomic profiling of cellular responses to NLRP3 agonists

Louis Robert Hollingsworth, Priya Veeraraghavan, João A. Paulo, J. Wade Harper

This study profiles proteomic changes in subcellular compartments during NLRP3 inflammasome activation and identifies disruptions in retrograde trafficking pathways.

bioRxiv · Apr 19, 2024 · PreprintCell Biology