Multiplex PCR method for MinION and Illumina sequencing of Zika and other virus genomes directly from clinical samples
Joshua Quick, Nathan D. Grubaugh, Steven T. Pullan, Ingra Morales Claro, Andrew D. Smith, Karthik Gangavarapu, Glenn Oliveira, Refugio Robles‐Sikisaka, Thomas F. Rogers, Nathan Beutler, Dennis R. Burton, Lia Laura Lewis‐Ximenez, Jaqueline Góes de Jesus, Marta Giovanetti, Sarah Catherine Hill, , Nicholas James Loman
This paper describes a method to sequence Zika and other virus genomes directly from clinical samples using multiplex PCR and portable sequencing technologies, achieving complete genomes in 1-2 days.
Nature Protocols · May 24, 2017Peer-reviewedGenomics