This study uses Carbotrace 480 to detect protoplasts in a pigment-deficient mutant of Chlorella sorokiniana, identifying Snailase as the most effective enzyme for cell wall degradation.

bioRxiv · Aug 28, 2026 · PreprintBioengineering

An activity-resistance tradeoff constrains enzyme evolution

Alexander W. Sarkis, Jens Laurids Sørensen, Teis Esben Søndergaard, Kåre Lehmann Nielsen, Jens C. Frisvad, Douglas L. Theobald, Lizbeth Hedstrom

This paper investigates the evolutionary tradeoff between enzyme activity and resistance to mycophenolic acid (MPA) and finds that resistance correlates with reduced catalytic efficiency across both ancestral and extant enzymes.

Proceedings of the National Academy of Sciences · Jul 23, 2026Peer-reviewedEvolutionary Biology

Non-ribosomal Peptides as Structural Determinants of Fungal Hydrophobicity

Trine Aalborg, Klaus Ringsborg Westphal, Balazs Delenyi, Thea Laulund Lunden, Marie O Joergensen, Dmitry A. Tolmachev, Trine Sørensen, Maria Sammalkorpi, Jens L Soerensen, Peter Kjær Kristensen, Herbert Kogler, Markus B. Linder, Reinhard Wimmer, Teis Esben Søndergaard

The paper identifies WARPs, cyclic non-ribosomal peptides, as structural determinants of fungal hydrophobicity, showing their role in maintaining surface hydrophobicity and aerial hyphae integrity.

bioRxiv · Jul 13, 2026 · PreprintMicrobiology