James P. B. Lloyd

PICARD-seq enables rapid, affordable whole-plasmid sequencing using nanopore technology, improving accuracy and resolving repetitive regions in synthetic constructs.

The paper identifies context-specific failures of the Cre recombinase in plant gene circuits and develops improved recombinase systems for stable memory functions.

The paper shows that using authentic start codons improves NMD target identification and protein structure prediction in Arabidopsis thaliana.

This paper develops CRISPRi-based gene circuits in Arabidopsis for implementing logic gates and modular control of gene expression.

The paper introduces synthetic gene circuits in plants that enable programmable reconfiguration of transcriptional activity through logic gates and genetic recombination.

The paper identifies NMD-targeted transcripts in human cells by analyzing RNA-Seq data from NMD-inhibited HeLa cells, revealing broad regulatory potential of alternative splicing coupled with NMD.

The paper characterizes a regulatory network of splicing factors through alternative splicing coupled with NMD, revealing extensive interactions and NMD targeting of splicing factors.

The paper uses polysome fractionation data to identify features of NMD-targeted transcripts, finding the EJC model is a strong predictor while long 3 UTRs are not.