Christian Pflueger

PICARD-seq enables rapid, affordable whole-plasmid sequencing using nanopore technology, improving accuracy and resolving repetitive regions in synthetic constructs.

The paper identifies context-specific failures of the Cre recombinase in plant gene circuits and develops improved recombinase systems for stable memory functions.

The paper introduces a modular dCas9-based platform for combinatorial epigenome editing, demonstrating its ability to control effector recruitment and spatial organization for improved transcriptional regulation.

This paper develops CRISPRi-based gene circuits in Arabidopsis for implementing logic gates and modular control of gene expression.

The paper introduces synthetic gene circuits in plants that enable programmable reconfiguration of transcriptional activity through logic gates and genetic recombination.

This paper develops a modular dCas9-SunTag DNMT3A system to improve DNA methylation editing specificity and reduce off-target effects compared to direct fusion constructs.