Ambika Bhagi‐Damodaran

Rational design of tertiary coordination sphere of a heme-based sensor for two-orders enhanced oxygen affinity

This study enhances the oxygen affinity of the DosS protein by modifying its heme coordination sphere, achieving a 150-fold increase in O2 binding affinity.

Sequential oxidation of L-lysine by a non-heme iron hydroxylase

The paper demonstrates sequential oxidation of L-lysine by a non-heme iron hydroxylase and computationally analyzes structural elements that enable this reactivity.

Machine learning guided rational design of a non-heme iron-based lysine dioxygenase improves its total turnover number

This paper uses machine learning and molecular dynamics to design improved variants of a non-heme iron-dependent lysine dioxygenase, achieving higher turnover numbers.

Controllable multi-halogenation of a non-native substrate by SyrB2 iron halogenase

This paper demonstrates how SyrB2 iron halogenase can controllably halogenate non-native substrates to produce mono-, di-, and tri-chlorinated products by adjusting cofactor loading and electron transfer.

Equilibrium dialysis with HPLC detection to measure substrate binding affinity of a non-heme iron halogenase

The paper describes a method using equilibrium dialysis and HPLC to measure the substrate binding affinity (Kd) of the non-heme iron halogenase BesD for lysine.