Boon Huan Tan

Evidence for a biphasic mechanism of virus transmission of the human metapneumovirus in LLC-MK2 cell monolayers.

This study shows HMPV transmission in LLC-MK2 cells occurs in two phases: initial cell-to-cell spread via virus filaments, followed by cell-free virus spread linked to membrane changes and signaling pathway activation.

Evaluating the use of siRNA to silence the expression of the H5N2 virus polymerase genes as strategy to block the transmission of the avian H5N2 virus in mammalian cells.

This study evaluates siRNA targeting H5N2 virus polymerase genes to block virus transmission in mammalian cells, finding that effective siRNAs reduce mRNA levels and inhibit viral replication.

Analysis of the surface topology of respiratory syncytial virus particles that form on the surface of virus-infected cells.

This study uses electron and atomic force microscopy to image the surface topology of RSV filaments on infected cells, revealing structured domains and glycocalyx involvement.

Evidence that the cell glycocalyx envelops respiratory syncytial virus (RSV) particles that form on the surface of RSV-infected human airway cells.

The paper shows that the glycocalyx envelops RSV particles as they form on infected cells, with HS and SYND4 on the virus surface.

Evidence that the SARS-CoV-2 S protein undergoes a conformational change at the Golgi Complex that leads to the formation of virus neutralising antibody binding epitopes in the S1 protein subunit.

The study shows that SARS-CoV-2 S protein undergoes a conformational change at the Golgi complex, forming epitopes recognized by virus-neutralizing antibodies in the S1 subunit.