Nicholas M. Tefft

This paper demonstrates a modular platform for enzyme encapsulation using bacterial microcompartment shells, showing successful activity and stability for multiple enzymes.

This study shows that encapsulating the enzyme TPI in HO BMC shells improves its thermal stability up to 62°C.

The paper studies non-native cargo loading into HO shell variants and finds that activity depends on cargo amount, not shell permeability, with simpler variants showing robust assembly.

The paper uses SAXS and PDDF to analyze BMC shell structures and enzyme encapsulation in Haliangium ochraceum BMCs expressed in E. coli, showing cytoplasmic protein capture during assembly.

The paper identifies a new function of an enzyme in bacterial microcompartments, proposing NAD+ regeneration and a role for the shell in redox separation.

The paper engineers an inward electron transfer pathway in Shewanella oneidensis to generate NADH using electrode-driven electrons and a light-driven proton pump.