Cheryl A. Kerfeld

Multi-lab, Multi-enzyme Study Demonstrates the Versatility of Bacterial Microcompartment Shells as a Modular Platform for Confined Biocatalysis

This paper demonstrates a modular platform for enzyme encapsulation using bacterial microcompartment shells, showing successful activity and stability for multiple enzymes.

The new phycobilisome linker protein ApcI regulates high light adaptation in Synechocystis sp. PCC 6803

This study identifies ApcI as a phycobilisome linker protein in Synechocystis sp. PCC 6803 that regulates high light adaptation by replacing ApcG and interacting with photosystem II.

Structure Characterization of Bacterial Microcompartment Shells via X-ray Scattering and Coordinate Modeling: Evidence for adventitious capture of cytoplasmic proteins

The paper uses SAXS and PDDF to analyze BMC shell structures and enzyme encapsulation in Haliangium ochraceum BMCs expressed in E. coli, showing cytoplasmic protein capture during assembly.

Electrochemical cofactor recycling of bacterial microcompartments

The paper identifies a new function of an enzyme in bacterial microcompartments, proposing NAD+ regeneration and a role for the shell in redox separation.

A chaotrope-based approach for rapid in vitro assembly and loading of bacterial microcompartment shells

This paper introduces a chaotrope-based method for in vitro assembly of bacterial microcompartment shells, enabling controlled encapsulation of cargo and expanding design possibilities.

Orthogonality of shell proteins across BMC subclasses in cyanobacteria

The paper investigates cross-reactivity between shell proteins of different BMC subclasses in cyanobacteria and identifies strategies to enhance orthogonality.