Resolving phylogenetic and biochemical barriers to functional expression of heterologous iron-sulphur cluster enzymes
Francesca D’Angelo, Elena Fernández‐Fueyo, Pierre Simon Garcia, Helena Shomar, Martin Pelosse, Rita Rebelo Manuel, Ferhat Büke, Siyi Liu, Niels J. F. van den Broek, Nicolas Duraffourg, Carol de Ram, Martin Pabst, Emmanuelle Bouveret, Simonetta Gribaldo, Béatrice Py, , Gregory Bokinsky
The paper investigates why iron-sulphur cluster enzymes are often inactive when heterologously expressed in E. coli and identifies strategies to improve their functionality.
eLife · Feb 4, 2022Peer-reviewedSynthetic Biology