Shotgun scanning glycomutagenesis: a simple and efficient strategy for constructing and characterizing neoglycoproteins
Mingji Li, Xiaolu Zheng, Sudhanshu Shanker, Thapakorn Jaroentomeechai, Tyler D. Moeller, Sophia W. Hulbert, İlkay Koçer, Josef Byrne, Emily C. Cox, Qin Fu, Sheng Zhang, Jason W. Labonte, Jeffrey J. Gray, Matthew P. DeLisa
This paper introduces a method to construct and analyze neoglycoproteins by systematically modifying glycosylation sites in proteins and assessing the effects of glycan addition.
Proceedings of the National Academy of Sciences · Sep 22, 2021Peer-reviewedSynthetic Biology