Mingji Li

The paper describes a method to isolate full-length IgG antibodies from combinatorial libraries in E. coli using a bifunctional antigen substrate for selection.

The paper evolves Escherichia coli Tat translocases to enhance protein secretion, finding three variants that significantly increase periplasmic protein export compared to wildtype and SecYEG.

This paper introduces a method to construct and analyze neoglycoproteins by systematically modifying glycosylation sites in proteins and assessing the effects of glycan addition.

This paper describes the engineering of ubiquibodies (uAbs) that degrade all forms of endogenous ERK1/2 protein kinase in multiple cell lines.