BiochemistrybioRxivPreprint
High-fidelity CRISPR genome editing of single-nucleotide mutation with near-complementary guide RNA via enhanced target binding kinetics
Hyomin K. Lee, Seohyun Kim, Hyeon Jong Yu…·Junho K Hur
This paper introduces a CRISPR-Cas9 editing strategy using near-complementary sgRNAs to specifically target single-nucleotide mutations without relying on PAM sequences, demonstrating its effectiveness on a cancer-related mutation.