Chang Ho Sohn

Fixative eXchange (FX)-seq: Scalable Single-nucleus RNA Sequencing Analysis of PFA-fixed or FFPE Tissue

FX-seq improves snRNA-seq from FFPE and PFA-fixed tissues by removing crosslinks and preventing RNA leakage, enabling analysis of 321,710 nuclei from mouse and human samples.

High-fidelity CRISPR genome editing of single-nucleotide mutation with near-complementary guide RNA via enhanced target binding kinetics

This paper introduces a CRISPR-Cas9 editing strategy using near-complementary sgRNAs to specifically target single-nucleotide mutations without relying on PAM sequences, demonstrating its effectiveness on a cancer-related mutation.