Anila George

Systematic benchmark of demultiplexing workflows for direct RNA sequencing

This study benchmarks two demultiplexing tools for direct RNA sequencing using IVT spike-ins and species identity, showing their performance differences and the need for calibration.

Guide RNA truncation as a tunable approach for modulating base editing outcomes

This study shows that truncating guide RNAs can modulate base editing outcomes by reducing bystander editing while preserving on-target conversion in multiple contexts.

Editing of highly homologous gamma-globin genes by nickase deficient Base Editor mitigates large intergenic deletions

This study evaluates the safety of using dCas9-ABE8e for editing gamma-globin genes, showing reduced large deletions compared to nCas9-ABE8e while maintaining editing efficiency.

Precise modelling and correction of a spectrum of β-thalassemic mutations in human erythroid cells by base editors

This study demonstrates the use of base editors to precisely correct various β-thalassemic mutations in human erythroid cells, showing efficient and scarless mutation correction with normal hemoglobin variants.

Identification of novel HPFH-like mutations by CRISPR base editing that elevates the expression of fetal hemoglobin

This study uses CRISPR base editing to identify novel mutations in the HBG promoter that increase fetal hemoglobin expression without harmful effects.