Polysome-profiling in small tissue samples
Shuo Liang, Hermano Martins Bellato, Julie Lorent, Fernanda C. S. Lupinacci, Christian Oertlin, Vincent van Hoef, Victor P. Andrade, Martín Roffé, Laìa Masvidal, Glaucia N. M. Hajj, Ola Segnestam Larsson
This paper presents an optimized non-linear sucrose gradient method for polysome-profiling that reduces sample handling and enables translatome analysis from small tissue samples and primary cells.
Nucleic Acids Research · Oct 10, 2017Peer-reviewedMolecular Biology