Saturation mutagenesis genome engineering of infective {Phi}X174 bacteriophage via unamplified oligo pools and golden gate assembly
Matthew S. Faber, James T. Van Leuven, Martina M. Ederer, Yesol Sapozhnikov, Zoë L. Wilson, Holly A. Wichman, Timothy A. Whitehead, Craig R. Miller
The paper describes a protocol for generating saturation mutagenesis libraries of the {Phi}X174 bacteriophage using unamplified oligo pools and golden gate assembly, enabling comprehensive mutation analysis of viral proteins.
ACS Synthetic Biology · Dec 11, 2019Peer-reviewedGenomics