Holly A. Wichman

The paper evaluates predictive models for {Phi}X174 bacteriophage viability using biophysical and molecular modeling approaches, finding that large-effect mutations are highly informative for predicting phenotypes.

This study shows that prophages can drive observed host-range expansion in directed evolution experiments, emphasizing the need for sequencing to verify phage origins in phage therapy applications.

This study shows that systematically introducing synonymous mutations in bacteriophage {Phi}X174 reduces viral fitness, but the effects are non-linear and gene-dependent.

The paper describes a protocol for generating saturation mutagenesis libraries of the {Phi}X174 bacteriophage using unamplified oligo pools and golden gate assembly, enabling comprehensive mutation analysis of viral proteins.