GenomicsACS Synthetic BiologyPeer-reviewed
Saturation mutagenesis genome engineering of infective {Phi}X174 bacteriophage via unamplified oligo pools and golden gate assembly
Matthew S. Faber, James T. Van Leuven, Martina M. Ederer…·Craig R. Miller
The paper describes a protocol for generating saturation mutagenesis libraries of the {Phi}X174 bacteriophage using unamplified oligo pools and golden gate assembly, enabling comprehensive mutation analysis of viral proteins.
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