James T. Van Leuven

This study shows that phage resistance evolution is driven by ecological factors like competitive interactions and fitness costs, not mutation rates, and depends on phage and host concentrations.

This study evaluates PMA-qPCR for quantifying viable Paenibacillus larvae and Melissococcus plutonius in honeybee hive samples, showing reduced dead-cell detection but needing optimization at high cell densities.

This study identifies Bacillus species that enhance honeybee larval survival during Paenibacillus larvae infection and show potential for adult bee survival with minimal impact on gut microbiome.

The paper evaluates predictive models for {Phi}X174 bacteriophage viability using biophysical and molecular modeling approaches, finding that large-effect mutations are highly informative for predicting phenotypes.

This study models how protein stability affects fitness in {phi}X174 bacteriophage using deep mutational scanning and a multistage binomial model, finding that large destabilizing mutations predict inviability but smaller ones do not reliably predict survival.

This study shows that Paenibacillus larvae can rapidly evolve resistance to bacteriophages, but resistance often leads to growth defects and reduced virulence in bee larvae.

This study shows that prophages can drive observed host-range expansion in directed evolution experiments, emphasizing the need for sequencing to verify phage origins in phage therapy applications.

The paper modifies the Appelmans protocol to evolve phages against Paenibacillus larvae, identifying new lysis patterns and improving phage diversity for honey bee pathogen treatment.

This study shows that systematically introducing synonymous mutations in bacteriophage {Phi}X174 reduces viral fitness, but the effects are non-linear and gene-dependent.

The paper describes a protocol for generating saturation mutagenesis libraries of the {Phi}X174 bacteriophage using unamplified oligo pools and golden gate assembly, enabling comprehensive mutation analysis of viral proteins.