Klaus Pfeffer

APEX2 proximity labeling maps the mGBP2-proximal proteome at the Toxoplasma gondii parasitophorous vacuole

The paper uses APEX2 proximity labeling to identify mGBP2-associated proteins at the Toxoplasma gondii parasitophorous vacuole and finds COX2 is critical for parasite survival.

Characterization of a fungal mixed-linkage glucan synthase

The paper characterizes AfTFT1 as a fungal MLG synthase and identifies key structural determinants for MLG biosynthesis.

Barcode Crosstalk in ONT Multiplex Sequencing: Quantification and Mitigation Strategies

The paper quantifies barcode crosstalk in ONT multiplex sequencing and evaluates mitigation strategies across three protocols, finding that protocol C eliminates crosstalk completely.

Intra-Strain Genetic Heterogeneity in Toxoplasma gondii ME49: Oxford Nanopore Long-Read Sequencing Reveals Copy Number Variation in the ROP8-ROP2A Locus

The paper uses Oxford Nanopore sequencing to identify genetic variation within the T. gondii ME49 strain, revealing copy number changes in the ROP8-ROP2A locus.

TNF Superfamily Member 14 Drives Post-Influenza Depletion of Alveolar Macrophages Enabling Secondary Pneumococcal Pneumonia

This study identifies TNFSF14 as a driver of alveolar macrophage depletion after influenza, increasing susceptibility to pneumococcal pneumonia, and shows targeting this pathway can reduce disease severity.

MetaGut: Insights into gut microbiomes in stem cell transplantation by comprehensive shotgun long-read sequencing

MetaGut uses long-read shotgun metagenomics to analyze gut microbiome composition and antibiotic resistance genes in patients undergoing alloHSCT, revealing significant inter-individual and temporal variability.