The paper quantifies barcode crosstalk in ONT multiplex sequencing and evaluates mitigation strategies across three protocols, finding that protocol C eliminates crosstalk completely.

bioRxiv · Mar 28, 2026 · PreprintMolecular Biology

MetaGut: Insights into gut microbiomes in stem cell transplantation by comprehensive shotgun long-read sequencing

Philipp Spohr, Sebastian Alexander Scharf, Anna Rommerskirchen, Birgit Henrich, Paul Sebastian Jäger, Gunnar W. Klau, Rainer R. Haas, Alexander Dilthey, Klaus Pfeffer

MetaGut uses long-read shotgun metagenomics to analyze gut microbiome composition and antibiotic resistance genes in patients undergoing alloHSCT, revealing significant inter-individual and temporal variability.

Scientific Reports · Feb 19, 2024Peer-reviewedBioinformatics

Complete sequences of six Major Histocompatibility Complex haplotypes, including all the major MHC class II structures

Torsten Houwaart, Stephan Scholz, Nicholas R. Pollock, William Hunt Palmer, Katherine M. Kichula, Daniel Rodrigo Strelow, Duyen Bao Le, Dana Belick, Lisanna Hülse, Tobias Lautwein, Thorsten Wachtmeister, Tassilo Erik Wollenweber, Birgit Henrich, Karl Köhrer, Peter Parham, , Alexander Dilthey

The paper completes six MHC haplotypes, including DR1, DR4, and structurally variable C4 regions, improving reference panels for immunogenomics analysis.

HLA · Mar 18, 2023Peer-reviewedGenomics