Synthetic BiologyFungal Biology and BiotechnologyPeer-reviewed
Modulating CRISPR gene drive activity through nucleocytoplasmic localization of Cas9 in S. cerevisiae
Megan E. Goeckel, Erianna M. Basgall, Isabel C. Lewis…·Gregory C. Finnigan
This study uses an artificial gene drive system in budding yeast to show that appending nuclear localization signals to Cas9 can modulate nuclease activity and act as a molecular safeguard.
14 citations