Julio Berríos

Engineering serine metabolism to enhance AOX1 promoter self-induction in a formate dehydrogenase-deficient Komagataella phaffii

This study enhances AOX1 promoter self-induction in K. phaffii by engineering serine metabolism to increase formate production, improving recombinant protein productivity without methanol.

Sorbitol uptake and oxygen transfer shape AOX1 promoter induction under methanol-free conditions in Komagataella phaffii lacking formate dehydrogenase

This study shows that increased sorbitol metabolism reduces AOX1 promoter induction in K. phaffii, while lower oxygen transfer enhances it, affecting recombinant protein productivity.

Formate from THF-C1 metabolism induces the AOX1 promoter in formate dehydrogenase-deficient Pichia pastoris

The paper shows that formate from THF-C1 metabolism induces the AOX1 promoter in formate dehydrogenase-deficient Pichia pastoris, enabling self-induced protein expression without methanol.