Anthony G. Vecchiarelli

The paper develops fluorescent biosensors for cryogenic correlative light and electron microscopy to measure physiological conditions alongside structural data.

This study shows polyphosphate regulates carbon fixation and nucleoid structure in cyanobacteria by organizing spatial relationships between carboxysomes and the nucleoid.

This study develops modular cargo loading strategies for carboxysome-derived protein particles, showing that cargo loading and assembly can be controlled by recruitment strategy and cargo introduction order.

This study investigates the McdAB system in Halothiobacillus neapolitanus, revealing that McdA's ATPase activity is DNA-stimulated and that McdB displaces McdA from DNA, forming gradients to prevent carboxysome aggregation.

This study shows that the McdAB system can be used to engineer spatial control of various bacterial organelles in E. coli, enabling programmable positioning for synthetic biology applications.

The paper shows that nucleoid compaction maintains carboxysome organization in Synechococcus elongatus PCC 7942 when McdAB activity is downregulated.

This paper introduces a plasmid toolkit for expressing and purifying functional carboxysomes, showing that modifying specific genes affects their size and uniformity.

This study identifies key residues in McdB that modulate its interaction with McdA, revealing distinct roles for weak and strong interactions in carboxysome positioning and partitioning.

The paper identifies McdB as a protein that interacts with McdA to create oscillatory patterns on the nucleoid, enabling equidistant positioning of carboxysomes in cyanobacteria.

The paper investigates how MinE regulates MinD membrane interactions to drive oscillatory patterns in E. coli, refining a toggle switch model for pattern formation.