Christopher A. Azaldegui

The paper develops fluorescent biosensors for cryogenic correlative light and electron microscopy to measure physiological conditions alongside structural data.

This study investigates the McdAB system in Halothiobacillus neapolitanus, revealing that McdA's ATPase activity is DNA-stimulated and that McdB displaces McdA from DNA, forming gradients to prevent carboxysome aggregation.

The paper shows stress causes bacterial BR-bodies to switch from mRNA decay to storage by changing their physical state and function.

This study shows that the McdAB system can be used to engineer spatial control of various bacterial organelles in E. coli, enabling programmable positioning for synthetic biology applications.

The paper shows that nucleoid compaction maintains carboxysome organization in Synechococcus elongatus PCC 7942 when McdAB activity is downregulated.

This study identifies key residues in McdB that modulate its interaction with McdA, revealing distinct roles for weak and strong interactions in carboxysome positioning and partitioning.

The paper identifies polyphosphate as a scaffold for phase-separated condensates in bacteria and humans, showing its role in RNA turnover and translation.

This study investigates the photophysical properties of PAmKate to enhance cryogenic single-molecule imaging by improving emitter localization rates through a 2-color bleaching process.