Baptiste Darracq

The paper fine-tunes a CRISPRi screen in Vibrio cholerae to enable pooled genome-wide screening with minimal cytotoxicity and identifies 368 essential genes.

The paper identifies anti-phage defence systems encoded by sedentary chromosomal integron cassettes in Vibrio cholerae, showing 16 cassettes provide phage resistance.

The paper investigates how inverted sedentary chromosomal integrons (SCIs) in Vibrio cholerae recover their original orientation through homologous recombination, maintaining cassette stability and bacterial fitness.

The paper shows toxin-antitoxin systems regulate cassette recombination dynamics in chromosomal integrons by killing cells when excision rates are too high.

The paper demonstrates that integron integrase can insert cassettes outside classical att sites, leading to new recombination sites called attG and expanding integron roles in bacterial evolution.