Didier Mazel

Replication coordination marks the domestication of large extrachromosomal replicons in bacteria

This study identifies two categories of extrachromosomal replicons in bacteria and shows that large replicons replicate in sync with the chromosome and use its replication machinery for stable inheritance.

Coupling metabolic enhancement to plasmid spread enables programmable antimicrobial control

This paper develops conjugative plasmids that block antibiotic resistance plasmid spread by combining metabolic enhancement and plasmid spread mechanisms in microbial communities.

Evolutionary Constraints on RNA Polymerase Gene Positioning in the Genome of Fast-Growing Bacteria..

This study shows that positioning RNAP-encoding genes near the replication origin in fast-growing bacteria is essential for optimal growth and gene dosage effects.

Unraveling the prevalence and multifaceted roles of accessory peptide deformylases in bacterial adaptation and resistance

The paper identifies accessory PDFs in bacterial genomes that confer resistance to PDF inhibitors and are mobile, posing a challenge for antibiotic development.

The tRNA epitranscriptomic landscape and RNA modification enzymes in Vibrio cholerae

This paper maps tRNA modifications in Vibrio cholerae using chemical sequencing and gene deletions, revealing species-specific features and their role in translation and stress responses.

Identification and biosynthesis of xildivaline, a novel and widespread peptide deformylase inhibitor from Gammaproteobacteria

The paper identifies xildivaline, a novel peptide deformylase inhibitor produced by Gammaproteobacteria, and elucidates its biosynthesis and structural features.

Extracellular vesicle production and membrane uptake promote repair and antibiotic tolerance in E. coli

This study shows that extracellular vesicles in E. coli sequester and remove antibiotics, repair membranes, and enhance survival without conferring resistance.

Plasmid-chromosome transcriptional crosstalk in multidrug resistant clinical enterobacteria

The paper investigates plasmid-chromosome transcriptional interactions in multidrug-resistant bacteria, finding that a plasmid-encoded regulator drives chromosomal operon overexpression, enhancing bacterial fitness and carbapenem resistance dissemination.

The recombination efficiency of the bacterial integron depends on the mechanical stability of the synaptic complex

The paper investigates how the mechanical stability of the integron synaptic complex affects recombination efficiency in bacteria, finding a strong correlation between stability and efficiency.

Fine-tuning of a CRISPRi screen in the seventh pandemic Vibrio cholerae

The paper fine-tunes a CRISPRi screen in Vibrio cholerae to enable pooled genome-wide screening with minimal cytotoxicity and identifies 368 essential genes.

Sedentary chromosomal integrons as biobanks of bacterial anti-phage defence systems

The paper identifies anti-phage defence systems encoded by sedentary chromosomal integron cassettes in Vibrio cholerae, showing 16 cassettes provide phage resistance.

Belt and braces: two escape ways to maintain the cassette reservoir of large chromosomal integrons

The paper investigates how inverted sedentary chromosomal integrons (SCIs) in Vibrio cholerae recover their original orientation through homologous recombination, maintaining cassette stability and bacterial fitness.

Long-term evolution reveals the role of the circadian cycle in the environmental adaptation of cyanobacteria

The paper uses experimental evolution to show that circadian clock mutations in cyanobacteria enhance growth under continuous light by altering transcriptomic and metabolic responses.

Cassette recombination dynamics within chromosomal integrons are regulated by toxin-antitoxin systems.

The paper shows toxin-antitoxin systems regulate cassette recombination dynamics in chromosomal integrons by killing cells when excision rates are too high.

A new route for integron cassette dissemination among bacterial genomes

The paper demonstrates that integron integrase can insert cassettes outside classical att sites, leading to new recombination sites called attG and expanding integron roles in bacterial evolution.

Cassette recruitment in the chromosomal Integron of Vibrio cholerae

The paper investigates cassette recruitment in the chromosomal integron of Vibrio cholerae during conjugation and natural transformation, showing that horizontally transferred cassettes can be inserted and expressed.