Théophile Niault

This study identifies two categories of extrachromosomal replicons in bacteria and shows that large replicons replicate in sync with the chromosome and use its replication machinery for stable inheritance.

The paper fine-tunes a CRISPRi screen in Vibrio cholerae to enable pooled genome-wide screening with minimal cytotoxicity and identifies 368 essential genes.

The paper demonstrates that integron integrase can insert cassettes outside classical att sites, leading to new recombination sites called attG and expanding integron roles in bacterial evolution.