Nathalie Ropek

In chronically stimulated human T cells, suppressed oxidative metabolism and nucleotide loss accompany MAP2K4 activation. Inhibiting MAP2K4 (with the MKK4/7 inhibitor BSJ-04-122) restored tumor-cell killing in vitro.

A Cre-dependent ER-targeted TurboID knock-in mouse enables cell type-specific secretory proteomics, mapping adipocyte and hepatocyte responses to fasting, LPS and obesity, with B cells profiled at baseline.

This study develops chemical probes to target splicing factors in T cells, showing state-dependent loss of ITK via SF3B1 and demonstrating covalent ligandability of splicing regulators.