Improved methods and optimized design for CRISPR Cas9 and Cas12a homology-directed repair
Mollie S. Schubert, Bernice Thommandru, Jessica Woodley, Rolf Turk, Shuqi Yan, Gavin Kurgan, Matthew McNeill, Garrett R. Rettig
Empirical ssODN design rules for Cas9, Cas9 D10A and Cas12a HDR, plus end-modified donors and an NHEJ-inhibiting small molecule, raise HDR up to 10-fold.
Scientific Reports · Sep 30, 2021Peer-reviewedMolecular Biology✦ In-depth digest