Gavin Kurgan

UNCOVERseq is a workflow for sensitive off-target nomination in CRISPR-Cas editing, demonstrating superior performance across multiple modalities and systems.

PASTA quantifies chromosomal translocations from amplicon sequencing with high accuracy and sensitivity, outperforming existing methods in detecting rare rearrangements.

The paper analyzes CRISPR-Cas9 off-target mutations and translocations at single-cell and tissue-specific levels in mice, revealing context-dependent variability in editing outcomes.

This paper develops gene-edited tacrolimus-resistant Treg cells (FKBP12KO-Treg) for clinical use in transplant recipients, showing they maintain immunoregulatory function while resisting tacrolimus.

Superb-seq captures single-cell Cas9 edits and transcriptomes, revealing on- and off-target effects on gene expression in over 34,000 cells.

This study compares CRISPR off-target detection tools using ex vivo edited CD34+ HSPCs, finding that HiFi Cas9 reduces off-target sites and some methods outperform others in sensitivity and specificity.

Empirical ssODN design rules for Cas9, Cas9 D10A and Cas12a HDR, plus end-modified donors and an NHEJ-inhibiting small molecule, raise HDR up to 10-fold.

CRISPAltRations is a cloud-based tool that improves the analysis of CRISPR-induced DNA repair events by outperforming existing software in annotating indels and HDR events.

This study replaces the HBA1 gene with HBB in β-thalassemia-derived HSPCs using CRISPR, normalizing globin ratios and restoring functional hemoglobin.