Gábor Balázsi

Optimizing a CRISPR-Cas13d gene circuit for tunable target RNA downregulation with minimal collateral RNA cutting

This paper develops MONARCH gene circuits to optimize CRISPR-Cas13d for tunable RNA downregulation with minimal off-target effects in human and green monkey cells.

Bursty expression enhances reliability of messaging in gene networks

The paper shows that bursty gene expression maximizes mutual information and reduces entropy in gene networks, suggesting it enhances reliability of communication in gene regulatory systems.

Evolutionary regain of lost gene circuit function

The paper tests if yeast populations can regain lost function in a synthetic gene circuit by exposing mutants to drug and inducer, revealing adaptation scenarios with or without restoring the circuit's function.

Noise-Reducing Negative-Feedback Optogenetic Circuits in Mammalian Cells

The paper develops optogenetic circuits with negative feedback to reduce gene expression noise and control gene activity in mammalian cells.

Functional effects of heating and cooling gene networks

The paper investigates how heating and cooling affect gene network function in yeast, finding nonmonotone expression trends and temperature-induced bimodality in synthetic circuits.

Backward evolution from gene network dynamics

This study uses mathematical models and experiments to show that a mutation in a regulator gene can lead to a permanent decrease in population fitness due to interactions with gene regulatory network dynamics.