Minako Ueda

Apical3DTip: Elliptic Cross-section-based Reconstruction for the Embryo Initial Cell of Arabidopsis

The paper introduces Apical3DTip, a method for 3D and 4D analysis of Arabidopsis embryo initial cell morphology using elliptic cross-sections and coordinate normalization, enabling accurate geometric characterization without mesh reconstruction.

Temporal changes in surface tension guide the accurate asymmetric division of Arabidopsis zygotes

The paper shows that temporal changes in surface tension at the growing tip of Arabidopsis zygotes maintain cortical microtubule bands, which guide the formation of the preprophase band and ensure asymmetric cell division.

Ca2+ oscillations promote microtubule-band turnover and support tip growth in Arabidopsis zygotes

This study shows Ca2+ oscillations drive microtubule-band turnover and support tip growth in Arabidopsis zygotes through a feedback loop, distinct from actin-based tip growth mechanisms.

Two-step polar plastid migration via F-actin and microtubules ensures unequal inheritance during asymmetric division of Arabidopsis zygote

The paper shows that plastids in Arabidopsis zygote undergo two-step migration via F-actin and microtubules, leading to unequal inheritance during asymmetric division.

Title: KymoTip: High-throughput Characterization of Tip-growth Dynamics in Plant Cells

KymoTip is a tool for analyzing live imaging data of plant cell tip growth, enabling accurate quantification of cell dynamics and intracellular events despite fluctuations in position and shape.

A simple and versatile plasma membrane staining method for visualizing living cell morphology in reproductive tissues across diverse plant species

This paper introduces a plasma membrane staining method using FM4-64 to visualize living cell morphology in reproductive tissues of diverse plant species, enabling high-resolution imaging and analysis without transgenic lines.

Transgenerational polarity axis inheritance during Ceratopteris embryogenesis

This study shows that maternal tissues guide the orientation of the Ceratopteris embryo axis, aligning its root pole toward maternal rhizoids, promoting progeny establishment through a nurturing strategy.

Agent-Based Simulation of Cortical Microtubule Band Movement in the Arabidopsis Zygote

The paper uses an agent-based simulation to study cortical microtubule band movement in Arabidopsis zygotes, identifying self-organization processes and the role of directional cues.

Brassinosteroids function as the plant male and female reproductive hormone coordinating gene expression.

This study shows that brassinosteroids (BRs) function as reproductive hormones in Arabidopsis, regulating gene expression in both male and female reproductive tissues during fertilization.

Distinct localization patterns of actin microfilaments during early cell plate formation in plants through deep learning-based image restoration

The paper uses deep learning to restore high-resolution images of actin microfilaments in plant cells, revealing distinct localization patterns during early cell plate formation.

Deep learning-based cytoskeleton segmentation for accurate high-throughput measurement of cytoskeleton density

This study uses deep learning to segment cytoskeleton structures in plant cells, improving density measurements compared to traditional methods.

HD-ZIP IV genes are essential for embryo initial cell polarization in the radial axis initiation in Arabidopsis

This study identifies HD-ZIP IV genes as essential for radial axis formation in Arabidopsis by regulating apical cell polarization and division orientation.

A viscoelastic-plastic deformation model of hemisphere-like tip growth in Arabidopsis zygotes

The paper develops a viscoelastic-plastic model to simulate tip growth in Arabidopsis zygotes and identifies non-dimensional parameters that maintain a hemisphere-like shape.