Tetsuya Higashiyama

Calcium Dynamics During Pollen Tube Reception in Arabidopsis Ovules

The paper investigates calcium dynamics in Arabidopsis ovules during pollen tube reception, showing N-linked glycosylation is required for synergid cell calcium oscillations but not pollen tube reception itself.

A live-imaging system for Arabidopsis leaf primordia at early stages

This paper develops a live-imaging system to observe the basal region of early Arabidopsis leaf primordia for over 50 hours.

Extracellular calcium modulates pollen tube growth and guidance in Arabidopsis thaliana

This study shows extracellular calcium affects pollen tube growth and guidance in Arabidopsis by modulating intracellular calcium levels and directional growth.

Intermediate Evolutionary State of Motile Sperm and Pollen Tubes in the Extant Gymnosperm Cycas revoluta

This study identifies a molecular intermediate state in Cycas revoluta fertilization, showing cycad sperm retain motility features while pollen tubes lack angiosperm-like tip growth mechanisms.

Brassinosteroids function as the plant male and female reproductive hormone coordinating gene expression.

This study shows that brassinosteroids (BRs) function as reproductive hormones in Arabidopsis, regulating gene expression in both male and female reproductive tissues during fertilization.

Vesicle-Mediated Regulation of Pollen tube Growth in Torenia fournieri

This study investigates vesicle-mediated pollen tube growth in Torenia fournieri using BFA and AS-ODNs, showing their effects on cell wall morphology and gene expression.

Brassinosteroids and Flavonols Confer Temperature Stress Tolerance to Pollen Tube Germination and Growth

This study shows that brassinosteroids and flavonols enhance pollen tube germination and growth under temperature stress by maintaining ROS homeostasis.

HD-ZIP IV genes are essential for embryo initial cell polarization in the radial axis initiation in Arabidopsis

This study identifies HD-ZIP IV genes as essential for radial axis formation in Arabidopsis by regulating apical cell polarization and division orientation.

DROL1 subunit of U5 snRNP in the spliceosome is specifically required to splice AT--AC-type introns in Arabidopsis

This study identifies DROL1 as an Arabidopsis U5 snRNP subunit required for splicing AT-AC-type introns, showing its role in plant growth and development through RNA-Seq analysis of mutants.

CZON-cutter: a CRISPR-Cas9 system with multiplexed organelle imaging in a simple unicellular alga

The paper introduces CZON-cutter, a CRISPR-Cas9 system for genome editing and organelle visualization in Cyanidioschyzon merolae, enabling validation of essential gene functions.

Presynaptic MAST Kinase Controls Bidirectional Post-SynapticResponses to Convey Stimulus Valence in C. elegans

The paper shows that presynaptic MAST kinase in C. elegans controls bidirectional postsynaptic responses to encode thermal stimulus valence through opposing signals.