Author

Rojo V. Rakotoharisoa

University of OttawaORCID 0000-0002-6901-4529Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

Click to filter author papers

Biochemistry· 1 Evolutionary Biology· 1

Signature Assays, Organisms & Methods

Click to filter · or open tag

Approach:experimental assay· 2 Assay:long-read sequencing· 1 Research Focus:protein folding & dynamics· 1

Frequent Co-Authors

Roberto A. ChicaiD· 2 shared Cindy KlausiD· 1 shared Frank OechsliniD· 1 shared Hiva DoustmohammadiiD· 1 shared James S. FraseriD· 1 shared Janet H. SancheziD· 1 shared Niayesh ZarifiiD· 1 shared Pooja AsthanaiD· 1 shared

Activity by Year

2025-2026 · peak 1/yr

Co-Author Collaboration Network

10 top collaborators · 31 mutual co-author links

Click any collaborator node to inspect or filter shared papers

Focal author (center)Selected co-authorORCID co-authorUnlinked name

Curved chords = mutual co-authorship

2 shared papersEvolutionary Biology

Roberto A. ChicaORCID

Mutual co-authors in cluster

Author profile →

2 papers

Recent·Most cited

Predictable adaptive evolution of a phage endolysin through substrate recognition optimization

Xiaojun Zhu, Vincent Somerville, Rong Shi, Rojo V. Rakotoharisoa, Roberto A. Chica, Sylvain Moineau, Frank Oechslin

This study shows how endolysins adapt predictably through mutations that enhance substrate recognition, despite functional trade-offs and some variability in evolutionary paths.

bioRxiv · May 11, 2026 · PreprintEvolutionary Biology

Distal mutations enhance catalysis in designed enzymes by facilitating substrate binding and product release

Niayesh Zarifi, Pooja Asthana, Hiva Doustmohammadi, Cindy Klaus, Janet H. Sanchez, Serena E. Hunt, Rojo V. Rakotoharisoa, Sílvia Osuna, James S. Fraser, Roberto A. Chica

This study shows that distal mutations improve enzyme catalysis by enhancing substrate binding and product release, working with active-site mutations to increase overall activity.

Nature Communications · Sep 30, 2025Peer-reviewedBiochemistry