Eva C. M. Nowack

A step toward synthetic endosymbiosis: introducing Escherichia coli and the trypanosomatid endosymbiont Candidatus Kinetoplastibacterium crithidii into mammalian cells using a polyethylene glycol-based fusion protocol

This paper introduces a PEG-based protocol to deliver Escherichia coli and Candidatus Kinetoplastibacterium crithidii into mammalian cells without genetic modification or specialized equipment.

The Paulinella chromatophore transit peptide part2 adopts a structural fold similar to the γ-glutamyl-cyclotransferase fold

The paper determines the structure of crTPpart2 from two chromatophore-targeted proteins, revealing a fold similar to γ-glutamyl-cyclotransferase and suggesting its role in protein import.

T7 RNA polymerase-based gene expression from a transcriptionally silent rDNA spacer in the endosymbiont-harboring trypanosomatid Angomonas deanei

The paper develops a new conditional gene expression system in Angomonas deanei using T7 RNA polymerase to enable genetic manipulations without interfering with endogenous transcription.

A novel host-encoded protein, ETP2, plays an important role in endosymbiont division in the trypanosomatid Angomonas deanei

The paper identifies ETP2, a host-encoded protein essential for endosymbiont division in Angomonas deanei, showing its localization and functional role in division and segregation.