Jörg A. Bachmann

The paper uses single-cell RNA-sequencing and immunoprofiling to identify two transcriptionally distinct basophil populations in human peripheral blood.

The paper compares three methods for long-read sequencing of 10x Genomics single-cell cDNA libraries to evaluate their performance in full-length transcript capture, barcode identification, and isoform detection.

The paper optimizes RNA input and adapter dilution strategies to reduce adapter dimers in miRNA sequencing using the QIAseq miRNA Library kit, improving data quality.