Orlando Contreras‐López

Convergent and lineage-specific genomic changes shape adaptations in sugar-consuming birds

The paper identifies convergent and lineage-specific genomic changes in sugar-feeding birds through genome and transcriptome analyses, revealing adaptive mechanisms in metabolic pathways.

Tech Note: Simplified protocol for SMARTer Pico kit

This paper simplifies the SMARTer Pico library prep protocol by reducing fragmentation time and skipping fragmentation, showing minimal impact on data quality and complexity.

Tech Note - Adaptive Sampling: targeted Oxford Nanopore long-read sequencing

This paper evaluates adaptive sampling in Oxford Nanopore long-read sequencing, finding it increases ROI coverage but shows positional effects.

Isoform-Level Analysis of 10x Genomics Single-Cell cDNA Libraries from Cultured K562 Cells Using Long-Read Sequencing

The paper compares three methods for long-read sequencing of 10x Genomics single-cell cDNA libraries to evaluate their performance in full-length transcript capture, barcode identification, and isoform detection.

Adapter dilution and input optimisation for Qiagen QIAseq miRNA Library kit

The paper optimizes RNA input and adapter dilution strategies to reduce adapter dimers in miRNA sequencing using the QIAseq miRNA Library kit, improving data quality.

Fragmentation of genomic DNA using Covaris g-Tubes prior to library preparation for sequencing

The paper evaluates DNA fragmentation using Covaris g-TUBEs at different centrifugal forces to optimize DNA size for Oxford Nanopore libraries.

Long-read whole genome analysis of human single cells

This study uses long-read sequencing to analyze the genomic architecture of single human cells, revealing high-resolution genetic variation and structural variants.