Jean‐Charles Walter

Fast assembly and in vivo coalescence of ParBF biocondensates involved in bacterial DNA partition

This study investigates ParBF biocondensate dynamics in bacteria, showing they maintain stability through regulated phase separation and ParAF's dual role in assembly and coalescence.

The ParB-CTP Cycle Activates Phase Separation in Bacterial DNA Segregation

The paper shows the ParB-CTP cycle enables phase separation in bacterial DNA segregation by enhancing ParB-ParB interactions, supported by simulations and experiments.

In vivo Assembly of Bacterial Partition Condensates on Circular Supercoiled and Linear DNA

This study investigates how DNA supercoiling affects ParB DNA binding and partition complex assembly in bacteria, finding no significant impact and suggesting ParB-ParB interactions are key for condensate formation.

Multiplexed chromatin imaging reveals predominantly pairwise long-range coordination between Drosophila Polycomb genes

The paper uses multiplexed chromatin imaging to show that Drosophila Polycomb genes predominantly form pairwise long-range interactions rather than clustering during development.

Physical modeling of a sliding clamp mechanism for the spreading of ParB at short genomic distance from bacterial centromere sites

The paper models the ParB sliding clamp mechanism for DNA spreading and finds it cannot fully explain in vivo binding patterns and rapid assembly kinetics.

Robust and conserved stochastic self-assembly mechanism for dynamic ParB-parS partition complexes on bacterial chromosomes and plasmids

The paper identifies the Nucleation & Caging model as the mechanism for ParB-parS partition complex formation, showing it is conserved across plasmids and chromosomes.