Jean-Bernard Fiche

Nanoscale regulation of ROS signaling at the plasma membrane tunes the plant response to osmotic stress

This study shows that localized H2O2 nanodomains at the plasma membrane regulate ROP6 clustering and plant response to osmotic stress.

Mechanisms controlling the plasma membrane targeting and the nanodomain organization of the plant SPFH protein HIR2

This study identifies S-acylation and oligomerization as key factors controlling HIR2 plasma membrane targeting and nanodomain organization in Arabidopsis.

Sphingolipid-driven interleaflet coupling orchestrates Rho-GTPase recruitment to nanodomains for signal activation in plants

The paper shows sphingolipid-driven interleaflet coupling organizes nanodomains and activates Rho-GTPase in plants, linking membrane asymmetry to signaling.

Near-critical chromatin fluctuations facilitate long-range contacts in Drosophila chromosomes

This study shows that near-critical chromatin dynamics in Drosophila embryos best explain experimental data on long-range contacts and structural organization.

Single-allele chromatin folding is organized by recurrent motifs that are reweighted across cell types and states

The paper shows that chromatin folding is organized by recurrent motifs that are reweighted across cell types and states, revealing a structured framework for chromatin variability.

Multiplexed chromatin imaging reveals predominantly pairwise long-range coordination between Drosophila Polycomb genes

The paper uses multiplexed chromatin imaging to show that Drosophila Polycomb genes predominantly form pairwise long-range interactions rather than clustering during development.

pyHiM, a new open-source, multi-platform software package for spatial genomics based on multiplexed DNA-FISH imaging

pyHiM provides a modular, open-source software package for analyzing spatial genomics data from multiplexed DNA-FISH imaging, enabling chromatin trace reconstruction and data validation.

3D chromatin interactions involving Drosophila insulators are infrequent but preferential and arise before TADs and transcription

The paper investigates Drosophila insulators' role in 3D chromatin organization, finding that non-border preferential interactions occur before TADs and transcription.

Cis-regulatory chromatin loops arise before TADs and gene activation, and are independent of cell fate during development

The paper uses Hi-M to study cis-regulatory loops in Drosophila development, showing they form before TADs and gene activation, and are independent of cell fate.