O-GlcNAcylation and an importin-β radial gradient keep the FG barrier liquid in live-cell nuclear pores
Miao Yu, Maziar Heidari, Karen Palacio‐Rodríguez, Hao Ruan, Sara Mingu, Alam Ahmad Hidayat, Mathis Bode, Mateusz Sikora, Gerhard Hummer, Edward A. Lemke
The paper shows FG-NUPs in live cells exhibit liquid-like mobility, while in vitro they transition to solid, with O-GlcNAcylation and importin-β maintaining the dynamic FG network's liquidity.
bioRxiv · Dec 11, 2025 · PreprintBiophysics