Edward A. Lemke

A Phosphorylation-Induced Micellization switch in the low complexity domain of TDP-43

This study shows phosphorylation of TDP-43's low-complexity domain shifts its self-assembly from macrophase separation to micellization, potentially protecting against pathological aggregation.

A Unifying Thermodynamic Model for Phase Separation and Aging of Biopolymers

The paper develops a thermodynamic model that unifies phase separation and β-sheet-based aging in intrinsically disordered proteins, validated using nucleoporin-98 variants.

O-GlcNAcylation and an importin-β radial gradient keep the FG barrier liquid in live-cell nuclear pores

The paper shows FG-NUPs in live cells exhibit liquid-like mobility, while in vitro they transition to solid, with O-GlcNAcylation and importin-β maintaining the dynamic FG network's liquidity.

Direct RNA sequencing (RNA004) allows for improved transcriptome assessment and near real-time tracking of methylation for medical applications

This study evaluates improvements in direct RNA sequencing (DRS) using RNA004 chemistry and modified-base-calling models, showing enhanced throughput, accuracy, and detection of RNA modifications in clinical applications.

Differential conformational expansion of Nup98-HOXA9 oncoprotein in micro- and macrophases

This study examines the conformational changes of Nup98-HOXA9's FG domain across different assembly states, revealing micelle-like structures and dynamic transitions during condensate formation.