Author

Wayne A. Johnston

Queensland University of TechnologyORCID 0000-0002-7485-8363Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Synthetic Biology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 1 Organism:human· 1

Frequent Co-Authors

Elizabeth M. J. GillamiD· 1 shared James B. Y. H. BehrendorffiD· 1 shared Juan Alfaro-Palma· 1 shared Kirill A. AlexandroviD· 1 shared Raine E. S. ThomsoniD· 1 shared Robert E. SpeightiD· 1 shared

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2025-2025 · peak 1/yr

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6 top collaborators · 15 mutual co-author links

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1 shared paperSynthetic Biology

Elizabeth M. J. GillamORCID

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1 paper

Recent·Most cited

Synthetic BiologyACS Synthetic BiologyPeer-reviewedSep 15, 2025

Reconstitution of human cytochrome P450 activity using a Leishmania cell-free protein expression system

Wayne A. Johnston, Raine E. S. Thomson, Juan Alfaro-Palma…·James B. Y. H. Behrendorff

This study demonstrates the use of a Leishmania cell-free system to express and assay human P450 enzymes 3A4 and 2D6, showing distinct reductase preferences and drug interaction consistency.

2 citations

humanexperimental assay

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