Raine E. S. Thomson

Reconstitution of human cytochrome P450 activity using a Leishmania cell-free protein expression system

This study demonstrates the use of a Leishmania cell-free system to express and assay human P450 enzymes 3A4 and 2D6, showing distinct reductase preferences and drug interaction consistency.

Deep mutational scanning of CYP2C19 reveals a substrate specificity-abundance tradeoff

The paper uses deep mutational scanning to analyze CYP2C19 variants, revealing a tradeoff between substrate specificity and protein abundance.

Engineering indel and substitution variants of diverse and ancient enzymes using Graphical Representation of Ancestral Sequence Predictions (GRASP)

The paper introduces GRASP, a method for ancestral sequence reconstruction that handles large datasets and indels, demonstrating its ability to generate biologically active enzyme ancestors.