Nick Crang

Tech Note - Adaptive Sampling: targeted Oxford Nanopore long-read sequencing

This paper evaluates adaptive sampling in Oxford Nanopore long-read sequencing, finding it increases ROI coverage but shows positional effects.

Isoform-Level Analysis of 10x Genomics Single-Cell cDNA Libraries from Cultured K562 Cells Using Long-Read Sequencing

The paper compares three methods for long-read sequencing of 10x Genomics single-cell cDNA libraries to evaluate their performance in full-length transcript capture, barcode identification, and isoform detection.

Fragmentation of genomic DNA using Covaris g-Tubes prior to library preparation for sequencing

The paper evaluates DNA fragmentation using Covaris g-TUBEs at different centrifugal forces to optimize DNA size for Oxford Nanopore libraries.

Protein allocation and utilization in the versatile chemolithoautotroph Cupriavidus necator

This study investigates how Cupriavidus necator reallocates its proteome under different growth conditions and finds that many proteins are not utilized and autotrophy-related enzymes are underutilized.