Elton Paul Hudson

The paper screens variants of a Form II Rubisco to improve oxygen tolerance, identifies key mutations, and uses a transformer model to guide engineering.

The paper quantifies protease-mediated degradation of heterologous enzymes in cyanobacteria and compares expression between E. coli and Synechocystis.

The paper uses computational methods to redesign β-lactoglobulin, improving its stability and BCAA content, with experimental validation of the results.

This study identifies ppGpp-binding proteins in plants and cyanobacteria using chemoproteomics, revealing novel regulatory targets and conserved mechanisms compared to E. coli.

This study shows that immobilizing cyanobacteria in a nanocellulose matrix shifts their proteome from growth to sucrose synthesis by downregulating ribosomal proteins and upregulating carbon concentrating machinery and stress responses.

This study investigates the metabolic and physiological effects of L-lysine on Synechocystis sp. PCC 6803, identifying key genes involved in Lys resistance and metabolic disruption.

The paper develops a cyanobacterial platform to screen Rubisco mutants, identifying a variant with improved thermostability and resilience to detrimental exchanges.

This study investigates the energy metabolism of Cupriavidus necator under different trophic conditions by analyzing gene fitness using a barcoded transposon knockout library, revealing substrate-specific enzyme utilization and gene redundancy.

This study shows chloroplastic ascorbate modulates plant metabolism through global metabolome rewiring and enzyme interactions, independent of oxidative stress.

The paper identifies metabolite-protein interactions in the Calvin cycle of cyanobacteria and lithoautotrophic bacteria, revealing species-specific regulation and feed-forward activation mechanisms.

The paper screens CRISPR interference mutants in Synechocystis sp. PCC 6803 across trophic conditions, revealing tradeoffs between growth rate and robustness and identifying condition-specific gene functions.

This paper introduces an inducible CRISPR/Cas9 system for precise, markerless genome editing in Synechocystis sp. PCC 6803, enabling multiplexed edits and efficient segregation of mutants.

This study engineered cyanobacteria to convert CO2 into secreted short-to medium-chain hydrocarbons using synthetic metabolic pathways, achieving 230 mg/L of 1-alkene with 15% carbon partitioning.

This study investigates how Cupriavidus necator reallocates its proteome under different growth conditions and finds that many proteins are not utilized and autotrophy-related enzymes are underutilized.