Cryoprotectants-assisted plunge freezing of thick brain tissue specimens for targeted physiologically relevant cryo-imaging in situ
Ash Weier, Fei Gao, Elliot T Morgan…·Oleg V. Klykov
This paper develops a cryoprotectant-assisted plunge freezing method for preserving thick mouse brain tissue for cryo-imaging, enabling high-resolution visualization of cellular structures and validating their physiological relevance.